(Sorry, don't know if this has already been answered)
How that time were they able to measure (to verify the calculated data) the actual turning radius in flight? And how could one determine the altitude and speed where the tightest circle was possible?
It was
possible. It was also very difficult and only worked at low altitudes. You need ground observers (multiple) and modified surveying instruments. Ane there are limits.
There were also recording G meters (pens recording on paper strips/cylinders) but these did not line up very well with ground observations. That is ground observers didn't know what the airplane was pulling for Gs at any given time.
With a data base they could figure out if airplane was doing a 360 degree turn in 22 seconds and was doing about the same speed at the end as it was at the beginning (or of they knew the speed change) they could look at the charts and figure things out.
Not all planes (in fact most) tested did not have calibrated "test" instruments. Some the test instruments were big and heavy enough to require the removal or relocation of radios/other equipment.
Problem with actual test flights trying to measure the tightest radius is that they have to be done at low altitudes for the best accuracy (ground observers) and if the plane/pilot stalls how much room does he have to recovery before hitting the ground?
If the lower G tests were tracking with the calculated values that was probably good enough.
Stalling speeds
could be measured with cameras. Cameras were sometimes used for measuring landing distances. Again, once a data base had been established many flight tests were done without the elaborate test set ups. Unless something out of the expected started happening.
the 109 used a higher wing loading than the Spitfire (and Hurricane) and while the 109 did use more sophisticated high lift devices it was trying to make up a fair amount in wing loading.
109 needed to make up over 25% in coefficient of lift. That is badly stated, the 109s wing in total, needs to generate about 25% more lift than the Spitfire's wing. Not every sq ft of wing is generating the same amount of lift due to airfoil, and airflow over each part of the wing. But figuring that out is very hard.
2nd part is how much drag is wing making to generate the desired lift? If we are looking at CL of 1.3 and higher we have very high angles of attack.
We also have different parts of the wing operating under different conditions. Somebody has already mentioned the Spitfire wing having washout and the outer wing is not operating at the same angle of attack as the inner/mid wing and thus is not generating the same lift per sq ft. But once the 109 (or any other airplane) starts to low flaps to gain an increase that increase is only for the wing part/s that the flap is acting on. Not the area that the ailerons are located at. Unless we are dealing with drooping ailerons and extreme flap deflections.
The 109s slats do help but here again, they are not full span slats and only affect part of the wing. On the 109 only about 1/3 of square footage of wing is affected by the slats due to the wing taper. The Slats keep the outer wing from stalling. If they are fully out the inner wing is stalling and loosing lift.
Difference between airfoil tests in a wind tunnel and the plane actually flying. If you want the best coefficient of lift you need to juggle which parts of the wing are doing what because it may not be uniform across the span and for a 360 degree turn of around 20 seconds the high coefficient of lift means a much higher coefficient of drag.