No, this is more of a movie / video game thing. Certain guns or installations would definitely have noticeable flash in dark conditions, but not really in daylight.
A much more reliable indicator would be smoke streaming or puffing from guns firing.
A segment in this video demonstrates several things I've been talking about in this thread -- starting at 0:10
View: https://youtu.be/mNRbAO9CUhE?si=V3f9akscTAb_AeNC&t=10
Note:
- rounds from the Bf 109 with smoke phosphorous smoke trails (PmK or Phosphor mit Kern) bullets -- these are similar to British Mk.IV incendiaries
- rounds from the Bf 109 with less obvious smoke but emitting a flame from the base (SmK L'spur or Spitzgeschoß mit Kern Leuchtspur) bullets -- these are similar to British Mk.II tracers
- absence of any tracers from the Spitfire when it fires at the Ju 88
- visible smoke belching from the Spitfire's guns when it fires