Historical bombing altitudes were ~32000ft and 29000 ft (Nagasaki)
Lancaster VI service ceiling is 28500ft at 65000lb TO weight. However, at weapon release, the aircraft will have burnt off about 1500-1800 IG of fuel (~11000 to ~13000lb from 72000lb TO weight) and weight will be 61000-59000lb with a consequent increase in service ceiling. However even at 28000ft a Lancaster would have been safe from the ~21kt yield of a FAT MAN bomb as was proved by Bockscar dropping at ~29000ft. The LITTLE BOY bomb only had a yield of 13kt.
i think that's slightly optimistic.
I calculate a reduction in air density of 17% (ie 83%) between 23500ft and 28000ft which would reduce lift by 17%. In addition as the engines are above FTH the power would also drop 17%. A 12000lb reduction in weight from 72000lb to 60000lb is also a reduction of 17% however the reduction you refer to is from 65000lbs to 59000lbs is only 10%. There is a slightly reduction in parasitic drag. Although I can't see the operational ceiling (500 fpm) going from 23500 to 28000ft I can see it going to half way there, maybe 26000ft but the aircraft would reach 28000ft with maybe a climb rate of only 200fpm. This means the aircraft is hanging on the very edge edge of a stall with the engines at maximum power. This is a problem for an aircraft whose main escape was a corkscrew that generally lost enemy night fighters, The "silver plate" Lancaster VI would no doubt have fishpond for its H2S radar and village inn radar for its tail gunner and have the dorsal turret deleted. I think the Lancaster could drop little boy. Incidently the first atomic bomb to go off was little boy over hiroshima. There was no prototype test so yield could have been higher. Nitrous Oxide was used on single stage Merlin Mosquitos and we know the two stage used them so a nitrous oxide supply was a possibility. I think 26000ft is a good enough altitude and if done at night stood good chances of evading interception.
Air Pressure at Altitude Calculator
(assuming boyles law on PV = constant