The MkV which was tested which the injected 46 did 368 mph at 22,500, the report states the condition of the plane added 10 mph, a standard 45 engined MkV did 374 mph at 20,800ft, nothing was said about the condition. The data is from Boscome Down.
We really need to try to compare like to like as a lot of things are not adding up.
Yes, a MK V Spit with with a Merlin 45 did make 374-375mph at 20,800ft at Boscombe Down, however it was a MKVa with eight .303 guns and was operating at 6450lbs at take-off.
Spitfire Mk VA X.4922 Report
A later MK VB did 371mph at 20,100ft at 6525lbs and the drag of the cannon barrels/cannon drum fairings.
A tropical MK Vb did 354mph at 17,400ft at 6695lbs and with the cg 1in further back than the early Vb,
In addition to the tropical filter the plane's set up was "An aerial mast was fitted in the usual position on top of the fuselage, and I.F.F. aerials were fitted between the tail plane tips and the fuselage. An external mirror was fitted above the front of the hood. The aircraft was finished in desert camouflage. "
earlier tests make no mention of the radio mast, the IFF aerials or the external mirror one way or the other, they may have been fitted (the radio mast especially) or not.
The Spitfire performance site has no test of the Spitfire MK VI which is a MK V fitted with a Merlin 47 (Merlin 46 fitted with a cabin compressor for pressure cabin) the pressure cabin, a four bladed prop
and the extended wing tips.
However the book "The Spitfire story" by Alfred price gives test reports of both a Spitfire Va (tropical) fitted with a Merlin 46 (aircraft X4922), a MK VI (AB200) and a comparative tactical performance of a MK VI ((BR289) VS a standard MK Vb.
The Va tropical did 363mph at 20,800ft and 353 at 26,000ft at 6,440lbs
The MK VI did 356mph at 21,800ft and 343mph at 28,000ft at 6,740lbs
The MK VI in the in the comparative trial was (at 6,738lbs) was slightly slower below 20,000ft but 6mph faster at 22,000ft which was it's rated altitude.
Below 10,000ft it was bit slower in climb and and climbed at a less steep angle. from 10,000ft to 20,000ft there was little difference although the MK VI was slightly faster in climb. From 20,000 to 30,000ft the MK VI is about 1 minute faster than the test Vb. From 30,000ft to 35,000ft the MK VI takes half the time of the MK Vb.
Unfortunately no boost levels are given in any of these tests but the tactical trial was flown in May of 1942 with a plane from the middle of the production batch. The test with Aircraft AB200 was flown in Feb 1942 and AB 200 was the 2nd production MK VI and the test of the MK Va with Merlin 46 was flown in early 1942.
If combat with the Japanese over Darwin had been below 20,000ft then the Merlin 46s might very well have been part of the problem, bu if the combats were over 20,000ft then the problems were something other than the engines fitted.
edit, the Spit Va Tropical referred to was actually the same aircraft in the report linked to above but the tests were done about 8-9 months apart, the engine changed, the guns taken out and ballast used,and the gun ports sealed over. The same aircraft is reported to have made 355 1/2 mph at 20,800ft with a mock up of the 90 imp gallon jettisonable fuel tank and ballasted to 7,420lbs, max climb rate of 2050fpm at 14,600ft and service ceiling of 35,000ft.