The Me163 should have had one, but didn't although there is word that an engineer had been designing one for the Komet.
The He280 jet had the first ejection seats and a Heinkel test pilot was the first pilot to use an ejection seat, successfully ejecting from a He280 prototype during an emergency. The He219 also had ejection seats.
Unlike the He162, which used an explosive type system, the He280 and He219 used a compressed air system.